QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mattos, W. Articles by Palmquist, D. L. Search for Related Content PubMed PubMed Citation Articles by Mattos, W. Articles by Palmquist, D. L.

Biohydrogenation and Availability of Linoleic Acid in Lactating Cows^1,2,

Department of Dairy Science, Ohio Agricultural Research and Development Center, Wooster 44691

Linoleic acid biohydrogenation, absorption and availability for maintenance and milk production in dairy cows fed high grain (60–85% of dry matter) diets were quantitatively estimated by isotope dilution, using two methods of dosing. [1-¹⁴C]Linoleic acid-labeled chylomicra and very low density lipoproteins (VLDL) were obtained from lymph of a calf fed [1-¹⁴C]linoleic acid and fitted with a thoracic ductvenous shunt. Labeled chylomicra were injected intravenously into two cows: a Jersey (trial 1), and a Holstein (trial 2). Labeled VLDL was injected intravenously into a Holstein cow (trial 3). In trials 4 and 5 the [1-¹⁴C]linoleic acid was placed into the omasal canal of two rumen-fistulated Holstein cows. Linoleic acid biohydrogenation (%), absorption (g/day), and availability above requirements for milk production (mg/kg body wt^3/4) were: 68.1 ± 2.28, 52.1 ± 2.92, and 244 ± 19.4 (mean ± SE), respectively. The biohydrogenation data indicate that both methods of dosing the cows were equally dependable. The estimates of linoleic acid biohydrogenation are consistent with limited data previously reported, indicating that the isotope dilution technique used is a reliable method to estimate linoleic acid absorption in lactating cows. Linoleic acid available to the lactating cow above milk production requirements was more than double the requirement of weanling female rats, when compared on the basis of metabolic body size.

KEY WORDS: • biohydrogenation • linoleic acid • essential fatty acid • ruminants • lactation

¹ Supported in part by funds from USAID and by East Central Ohio Chapter, American Heart Association.

² Journal article No. 21-77, Ohio Agricultural Research and Development Center, Wooster.

³ Present address: Dept. De Zootecnia, E.S.A. "Luiz De Queiroz," Piracicaba, S.P., Brazil.

Manuscript received 21 February 1977.