QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shannon, B. Articles by Demos, P. Search for Related Content PubMed PubMed Citation Articles by Shannon, B. Articles by Demos, P.

Influence of Estrogen Treatment on Hepatic Cystathionine Synthase and Cystathionase of Female Rats Fed Varying Levels of Vitamin B-6^1,2,

Division of Biological Health, The Pennsylvania State University, University Park, Pennsylvania 16802

Diets containing 8, 4, 2, or 0 mg vitamin B-6/kg were fed to intact, female rats designated high-, medium-, low-, and deficient-diet groups, respectively. After receiving the diets for 2 weeks, each group was subdivided so as to receive scheduled subcutaneous injections of corn oil with or without estradiol benzoate (Es and NoEs rats, respectively). In experiment 1, rats were fed ad libitum and injections given daily for 2 weeks. In experiment 2, rats were pair-fed and injections given every other day for 3 weeks. Es injections contained 10 µg and 20 µg of estradiol benzoate in experiments 1 and 2, respectively. Hepatic cysthionine synthase (CStase) and cystathionase (CNase) activities were determined both with and without exogenous pyridoxal-5-phosphate (stimulated and basal activities, respectively). Regardless of estradiol treatment, the deficient-diet decreased food intake, weight gain, and the basal and stimulated activities of both enzymes with the exception of stimulated CNase activity in Es rats. Estradiol reduced stimulated CNase activity in the medium- and high-diet groups in experiment 1. In experiment 2, estradiol reduced basal CNase activity of all diet groups and reduced stimulated CNase of low- and high-diet groups. It had little effect on CStase activity.

KEY WORDS: • estrogen • cystathione synthase • cystathionase • vitamin B-6

¹ From The Pennsylvania State University, Agricultural Experiment Station and the College of Human Development, Division of Biological Health, University Park, Pennsylvania 16802.

² Authorized for publication on 11/08/76 as paper no. 5198 in the journal series of Pennsylvania Agricultural Experiment Station. Supported in part by Hatch Project 2037.

³ Current address: Box HC 398, University of Connecticut Health Center, Farmington, Connecticut 06032.

Manuscript received 8 December 1976.