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Effects of Selenium and Vitamin E Deficiencies in Lambs on Hepatic Microsomal Hemoproteins and Mitochondrial Respiration¹

Department of Agricultural Chemistry, Oregon State University, Corvallis, Oregon 97331

Microsomal hemoprotein levels and -amino levulinic acid dehydrase activity were determined in livers and rate of oxygen uptake, ADP:O ratios, and respiration control index were determined on mitochondria from muscle, liver, and heart of normal and white muscle diseased (WMD) lambs. WMD lambs were produced by feeding their dams either low selenium purified or alfalfa hay diets. Vitamin E and/or selenium was injected in a 2 x 2 factorial treatment in the ewes fed purified diets. Hepatic microsomal cytochrome P₄₅₀ levels and total heme content were significantly lower in WMD lambs. Cytochrome b₅ content was significantly lower in lambs on the -E - Se or -E + Se treatments than those on the +E - Se treatment, but the cytochrome b₅ content was not different between WMD and normal lambs from ewes fed the hay diet. No differences were found in hepatic -amino levulinic acid dehydrase activity, or in the rate of oxygen uptake, ADP:O ratios or respiratory control index between mitochondria from normal and WMD lamb tissue on any of the treatments.

KEY WORDS: • lambs • selenium • vitamin E • microsomal hemoproteins • mitochondrial respiration • -amino levulinic acid dehydrase

¹ Published with the approval of the Director of the Oregon State Agricultural Experiment Station as Technical Paper No. 4395. Supported by Public Health Service Research Grant NS 07413 from the National Institutes of Neurological Disease and Stroke.

Manuscript received 8 November 1976.