QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klain, G. J. Search for Related Content PubMed PubMed Citation Articles by Klain, G. J.

In Vivo Effects of Glucagon on Fatty Acid Synthesis in Fasted and Refed Rats^1,2,

Biochemistry Division, Department of Nutrition, Letterman Army Institute of Research, Presidio of San Francisco, California 94129

Since altered nutritional states evoke compensatory changes in systemic levels of several hormones, the present study was conducted to determine in vivo effects of glucagon and insulin on hepatic and adipose tissue lipogenesis in fed, fasted (3-days) and refed (3-days) rats. Compared to the fed controls, glucagon reduced hepatic fatty acid synthesis and acetyl CoA carboxylase activity by 62% and 65% in fed rats, and by 51% and 48%, respectively, in refed rats. In contrast, glucagon had no effect on fatty acid synthesis or acetyl CoA carboxylase activity in adipose tissue of any of the three experimental groups. Exogenous insulin antagonized the glucagon effects and restored hepatic fatty acid synthesis and enzyme activity in fed or refed rats. No glucagon or insulin effects were observed in fasting rats. In addition, glucagon reduced in vivo incorporation of acetate into hepatic cholesterol by about 33% and into fatty acids of the liver, and heart and the kidney by 33%, 77% and 30%, respectively. The hormone had no effect on fatty acid synthesis in the muscle.

KEY WORDS: • glucagon • insulin • lipogenesis • fasting • refeeding • liver • adipose tissue

¹ The opinions or assertions contained herein are the private views of the author and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.

² In conducting the research described in this report, the investigators adhered to the "Guide for Laboratory Animal Facilities and Care" as promulgated by the committee on the Guide for Laboratory Animal Facilities and Care of the Institutes of Laboratory Animal Resources, National Academy of Sciences-National Research Council.

Manuscript received 15 October 1976.

This article has been cited by other articles:

				W. Gu, H. Yan, K. A. Winters, R. Komorowski, S. Vonderfecht, L. Atangan, G. Sivits, D. Hill, J. Yang, V. Bi, et al. Long-Term Inhibition of the Glucagon Receptor with a Monoclonal Antibody in Mice Causes Sustained Improvement in Glycemic Control, with Reversible {alpha}-Cell Hyperplasia and Hyperglucagonemia J. Pharmacol. Exp. Ther., December 1, 2009; 331(3): 871 - 881. [Abstract] [Full Text] [PDF]