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Protection Against Carbon Tetrachloride-Induced Lipid Peroxidation in the Rat by Dietary Vitamin E, Selenium, and Methionine as Measured by Ethane Evolution^{1, 2,}

College of Agricultural and Life Sciences, Department of Biochemistry, University of Wisconsin-Madison, 420 Henry Mall, Madison, Wisconsin 53706

Dietary vitamin E, selenium (Se), and methionine were tested for their ability to inhibit carbon tetrachloride (CCl₄)-induced lipid peroxidation. Peroxidation, in vivo, was monitored by the evolution of ethane, an autoxidation product of -3-unsaturated fatty acids. Weanling rats were fed a basal diet low in vitamin E, Se, and sulfur-containing amino acids, or diets individually supplemented with these factors. After 3 to 7 weeks, the rats were injected with CCl₄ (ip) and ethane was collected for 9 hours. Cumulative ethane evolution was increased by CCl₄ in all groups. Vitamin E, Se, and methionine reduced ethane evolution from CCl₄-treated rats by 82%, 74%, and 60%, respectively. The toxicity of CCl₄ was decreased in correlation with ethane evolution. Thus, methionine and Se, probably by maintaining intracellular glutathione and glutathione peroxidase, protected against CCl₄-induced lipid peroxidation, as did vitamin E. Substitution of cod liver oil, which is rich in -3-unsaturated fat, for lard in the basal diet increased CCl₄-induced ethane evolution six-fold. Relative inhibition by the dietary supplements was not changed. Thus, the feeding of cod liver oil greatly increased ethane production which facilitated the detection and measurement of lipid peroxidation in vivo.

KEY WORDS: • lipid peroxidation • ethane • carbon tetrachloride • methionine • selenium

¹ A preliminary report of these experiments was presented at the 59th annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N.J., April 13-18, 1975. Hafeman, D. G. & Hoekstra, W. G. (1975) Protection by vitamin E and selenium against lipid peroxidation in vivo as measured by ethane evolution. Federation Proc. 34, 939 (abstr.).

² Research supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison, and by United States Public Health Service Program Grant no. AM 14881.

Manuscript received 9 March 1976.

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