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Effect of Folacin Deficiency on Folacin-binding Proteins in the Rat^1,2,

Biochemistry Research Laboratory, VA Hospital, Nashville, Tennessee 37203, and the Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

The effect of experimental folacin deficiency on the uptake and distribution of radioactive folic acid in the rat was investigated. Less radioactivity was taken up by livers of deficient rats than controls 24 hours after intraperitoneal injection of [³H]-folic acid, although more radioactivity was incorporated by the brain and kidneys of deficient rats. The distribution of radioactivity among the three folacin-binding proteins of rat liver cytosol and the binding protein of mitochondria was also studied. In deficiency, very little radioactivity was incorporated into cytosol binding proteins I and III, while more radioactivity was incorporated into cytosol binding protein II and the mitochondrial binding protein. A decrease in the endogenous folacin associated with all protein-bound and free forms was seen in deficiency with the major decrease coming at the expense of unbound folacin, and cytosol binding protein I. This latter protein may have a primary storage role in the liver.

KEY WORDS: • folacin • mitochondria binding protein

¹ Supported in part by the Medical Research Service of the Veterans Administration and Grants from the U.S. Public Health Service, No. AM-15289 and the Nutrition Foundation, No. 469.

² A preliminary report of this study was presented at the American Institute of Nutrition Meeting in April, 1976 (Federation Proc. 35, 581. 1976).

³ Taken in part from a thesis submitted to Vanderbilt University for the Ph.D. degree. Supported by the N.I.H. Predoctoral Training Grant No. 5T01-AM05441. Present address: Department of Biochemistry, University of Kansas Medical Center, Kansas City, Kansas 66103.

⁴ To whom requests for reprints should be addressed.

Manuscript received 12 January 1977.