QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Johnson, A. Articles by Kretchmer, N. Search for Related Content PubMed PubMed Citation Articles by Johnson, A. Articles by Kretchmer, N.

Adaptation of Rat Pancreatic Amylase and Chymotrypsinogen to Changes in Diet^1,2,

Department of Pediatrics, Division of Developmental Medicine, Stanford University School of Medicine, Stanford, California 94305

This study represents an attempt to determine the effect of dietary protein quality and hypophysectomy on the enzymic adaptability of the pancreas in the rat. The specific enzymes studied were amylase, which was purified by immunologic techniques, and chymotrypsinogen (activated), which was isolated by affinity column chromatography. Content and synthesis of each enzyme were accurately determined in relation to total pancreatic protein. When rats were fed a 64% sucrose diet (19% casein), there was a two- to three-fold increase in synthesis of amlyase. However, if a poor-quality protein (gelatin, gluten, or zein) was substituted for casein, there was no increase in the synthesis of amylase in response to increased carbohydrate. When rats were fed a 19% sucrose diet (64% casein), there was a significant increase in chymotrypsinogen synthesis. Of the poor-quality proteins, gluten was the only one effective in stimulating synthesis of chymotrypsinogen. Peptides, either free or as part of a protein, were necessary for the stimulation of chymotrypsinogen synthesis. Amylase synthesis in hypophysectomized rats was considerably depressed and unresponsive to increased carbohydrate. This effect could be partially relieved with hydrocortisone, corticosterone, or thyroxin, but not with growth hormone. Hypophysectomy had little effect on synthesis or content of chymotrypsinogen.

KEY WORDS: • pancreas • amylase • enzymatic adaptation • chymotrypsinogen

¹ Presented in part at the annual meeting of the Society for Pediatric Research, Washington, D.C., May, 1974. This investigation was supported in part by grants HD-00039, HD-02147, and HD-00391 from the National Institutes of Health.

² Some of these data were recorded in an M.D. thesis by Abiodun Johnson, University of Ibadan, Ibadan, Nigeria.

³ Present address: Department of Pediatrics, University College Hospital, Ibadan, Nigeria.

⁴ Address for reprints: Department of Pediatrics, Stanford University, Stanford, California 94305.

⁵ Present address: National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20014.

Manuscript received 2 April 1976.

This article has been cited by other articles:

				F. Zhao, S. S. Hou, H. F. Zhang, and Z. Y. Zhang Effects of Dietary Metabolizable Energy and Crude Protein Content on the Activities of Digestive Enzymes in Jejunal Fluid of Peking Ducks Poult. Sci., August 1, 2007; 86(8): 1690 - 1695. [Abstract] [Full Text] [PDF]