QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chee, H. Articles by Leveille, G. A. Search for Related Content PubMed PubMed Citation Articles by Chee, H. Articles by Leveille, G. A.

Influence of (—)-Hydroxycitrate on Lipogenesis in Chickens and Rats¹

Food Science and Human Nutrition Department, Michigan State University, East Lansing, Michigan 48824

The influence of (—)-hydroxycitrate on in vitro rates of fatty acid synthesis in chicken and rat liver and in rat adipose tissue was investigated. Following acute and chronic administration of (—)-hydroxycitrate to chickens and rats, changes in rates of fatty acid synthesis in vivo and in lipogenic enzyme activities were also determined. Addition of 1 mM (—)-hydroxycitrate to a buffer containing 10 mM glucose and liver slices from chickens or rats depressed the rate of ³H₂O incorporation into fatty acids. Acute administration of (—)-hydroxycitrate to chickens and rats also depressed in vivo rates of fatty acid synthesis in the livers of these species. Chickens appeared to be more sensitive to the inhibitory effects of (—)-hydroxycitrate than did rats. (—)-Hydroxycitrate was incorporated into the diets fed to the chickens and rats. Hepatic citrate cleavage enzyme activities were increased several fold in livers of chickens and rats fed (—)-hydroxycitrate. However, the rates of fatty acid synthesis in chicken and rat livers were depressed when expressed per g liver. Livers of chickens fed (—)-hydroxycitrate for 14 days were enlarged. Dietary (—)-hydroxycitrate did not depress rates of fatty acid synthesis per liver in these birds. Consumption of (—)-hydroxycitrate did not alter liver weight in rats. In vivo rates of fatty acid synthesis in rat adipose tissue were not influenced by consumption of a diet containing 52.6 mmoles (—)-hydroxycitrate/kg diet. Plasma triglyceride levels were increased two-fold in chickens, but unchanged in rats, fed (—)-hydroxycitrate for 2 to 3 weeks. There are species-specific as well as organ-specific responses to (—)-hydroxycitrate.

KEY WORDS: • (—)-hydroxycitrate • fatty acid synthesis • lipogenic enzymes • plasma triglycerides • liver • adipose tissue • chicken • rat

¹ Supported in part by Public Health Service Grants HL 14677 and AM 18957. DRR is the recipient of Career Development Award NIH K04 AM 00112. Michigan Agricultural Experiment Station Journal Article No. 7656.

Manuscript received 28 April 1976.

This article has been cited by other articles:

				A. P. Beigneux, C. Kosinski, B. Gavino, J. D. Horton, W. C. Skarnes, and S. G. Young ATP-Citrate Lyase Deficiency in the Mouse J. Biol. Chem., March 5, 2004; 279(10): 9557 - 9564. [Abstract] [Full Text] [PDF]