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Determination of Antitrypsin Activity on Agar Plates: Relationship between Antitrypsin and Biological Value of Soybean for Trout

Department of Poultry Science and Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853

A new method is described for determination of antitrypsin activity based on inhibition of trypsin solubilization of calcium caseinate in agar plates. The method was applied to analyze soybeans after graded heat treatments for their antitrypsin content. Biological determination of protein and energy values of the soybean samples showed direct correlation of these values with the destruction of antitrypsin as measured by the new method, using rainbow trout.

KEY WORDS: • antitrypsin • trypsin • soybeans • rainbow trout • protease inhibitors

¹ Research Fellow, Fulbright-Hayes Program: Department of Medicine, College of Veterinary Medicine, Helsinki, Finland.

Manuscript received 9 December 1975.