QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Leary, H. L. Articles by Lecce, J. G. Search for Related Content PubMed Articles by Leary, H. L., Jr. Articles by Lecce, J. G.

Uptake of Macromolecules by Enterocytes on Transposed and Isolated Piglet Small Intestine¹

Animal Science Department, North Carolina State University, Raleigh, North Carolina 27607

Previous work dealing with the internalization of macromolecules by enterocytes of neonatal piglet small intestine showed that the enterocytes of the proximal gut exhibited closure (ceased internalizing fluorescent protein) before those of the middle gut, which, in turn, exhibited closure before those of the distal gut. The rate of closure, however, could be manipulated by dietary regimen. The effect of digesta on closure as it relates to the position of the enterocyte in the digestive path was studied by either surgically transposing a portion of the ileum to the proximal third of the small intestine or isolating different regions of the small intestine from the direct digestive path. Transposition experiments illustrated that the neonatal piglet possesses a differentiated intestinal epithelium with regard to its capacity to internalize macromolecules. That is, enterocytes on ileum surgically transposed to the proximal intestine continued to internalize fluorescent protein after enterocytes on the proximal intestine on each side of the transposed ileum ceased. Upon isolation from the direct digestive pathway, enterocytes on intestinal segments from the proximal, middle, and distal thirds of the small intestine internalized fluorescent protein longer than comparable enterocytes remaining in the digestive path. It was concluded that digesta accelerates the rate of termination of the differentiated capacity of the neonatal piglet intestinal epithelium to internalize macromolecules.

KEY WORDS: • closure • enterocyte • intestine • macromolecule • neonate • pig

¹ Paper No. 4778 of the Journal Series of the North Carolina Agricultural Experiment Station, Raleigh. Supported in part by Grant CSRS No. 916-15-32, U.S.D.A., Cooperative State Research Service.

Manuscript received 9 September 1975.