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The Biotin Requirement of Rats Fed 20% Egg White¹

United States Department of Agriculture, Agricultural Research Service, Human Nutrition Laboratory, Grand Forks, North Dakota 58201

The biotin requirement of rats fed a diet based upon 62.4% sucrose, 20.0% egg white and 10.0% corn oil was determined using growth for 60 days as the criterion. Maximal growth and maximal concentration of cholesterol in plasma occurred when the diet contained 2.00 mg biotin/kg. Growth depression was noted in one of two experiments in which 3.00 mg of biotin was present per kg of diet.

KEY WORDS: • biotin requirement • egg white diets • plasma cholesterol

¹ Some of these data were presented at the 58th annual FASEB meeting, Atlantic City, New Jersey, April 8, 1974. An abstract was published: Klevay, L. M. (1974) The requirement for biotin of rats consuming a diet, 20% of which was egg white. Federation Proc. 33, 666.

Manuscript received 2 April 1976.