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-Aminoisobutyric Acid Transport in Liver Slices from Rats Fed Low Protein Meals^1,2,

Departments of Biochemistry and Nutritional Science, University of Wisconsin, Madison, Wisconsin 53706

The effect of low protein intake on -aminoisobutyric acid (AIB) transport has been examined in liver slices from meal-fed rats. Treatments (force-feeding casein or gelatin hydrolysates, glycine, or potassium chloride; injecting glucagon; or preincubating liver slices with cyclic AMP) which stimulated transport in control rats fed 3-hour meals containing 18% casein were less effective in rats fed 6% casein meals for 8 days. Responses of protein-depleted rats to glucagon or cyclic AMP had become essentially normal after they had consumed 18% casein meals for 2 days, whereas between 6 to 10 days were required for complete recovery of the response to casein hydrolysate. Stimulation of AIB transport by casein hydrolysate was also normal in depleted rats after they had consumed 40% casein meals for 2 days. Basal, non-stimulated transport of AIB was not significantly depressed in rats fed the low protein diet. Casein hydrolysate-induced increases in hepatic cyclic AMP concentrations were smaller in rats fed low protein meals. The results show that, although various stimuli of hepatic AIB transport become less effective in the protein-deprived rat, relatively rapid recovery of the response can occur upon refeeding adequate or high levels of protein.

KEY WORDS: • amino acid transport • dietary protein • cyclic AMP • glucagon

¹ This research was supported in part by the College of Agricultural and Life Sciences. University of Wisconsin, Madison, Wisconsin, and by a grant from the U.S. Public Health Service. No. AM 10747.

² A preliminary report was presented at the annual meeting of the Federation of American Societies for Experimental Biology, 1975, Federation Proc. 34, 882Abs.

Manuscript received 25 March 1976.