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Comparative Human Intestinal Bioavailability of Vitamin B-6 from a Synthetic and a Natural Source^1,2,3,

Nutrition Laboratory, Division of Gastroenterology, Department of Medicine, University of Florida, Gainesville, Florida 32610

Intraluminal perfusion of human jejunum has not been previously reported to demonstrate differences in bioavailability of vitamins from a food product. In this report, a triple lumen tube with a 30 cm study segment was used to study water soluble vitamin absorption in 15 normal subjects serving as their own controls. The intestinal uptake of vitamin B-6 from orange juice (O) and from a synthetic solution (S) perfused at 7.6 ml/minute were compared. In a separate series of perfusions in six subjects, the absorption of a mixture of pyridoxine, pyridoxal, and pyridoxamine from O and S solutions with and without glucose were compared. Mean vitamin B-6 absorption was significantly greater from S (65% uptake, transport rate 0.71 µg/cm/hour) than from O (30% uptake, transport rate 0.40 µg/cm/hour, p < 0.05). The addition of glucose to S enhanced this difference. Triple lumen intestinal perfusion can be used to discriminate differences in bioavailability of water soluble vitamins from natural and synthetic sources.

KEY WORDS: • vitamin B-6 • intestinal absorption • bioavailablity

¹ Supported by a grant from the Florida Citrus Commission. Lakeland, Florida.

² Presented in part at the Annual Meeting of the American Society for Clinical Nutrition, Atlantic City, N.J. 1975.

³ The experimental protocols received the administrative approval of the University of Florida Committee for the Protection of Human Subjects.

⁴ Address reprint requests to Edward W. Nelson, N.D., University of Florida College of Medicine, Box J-214. J. Hillis Miller Health Center, Gaineaville, Florida 32610.

Manuscript received 5 January 1976.