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Journal of Nutrition Vol. 106 No. 10 October 1976, pp. 1429-1432
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A Method to Measure the Adsorption of Bile Salts to Vegetable Fiber of Differing Water Holding Capacity

M. A. Eastwood, Rhoda Anderson, W. D. Mitchell, J. Robertson and S. Pocock1

Wolfson Gastrointestinal Laboratories, Western General Hospital, Edinburgh EH4 2XU, 1 Medical Computing and Statistics Group, University of Edinburgh, 17 Teviot Place, Edinburgh

Dietary vegetable fibers have physico-chemical properties which may influence their role in nutrition. A method is described which measures bile acid adsorption to fibers of differing water holding capacity. Such adsorption studies are complicated by the water held in the fiber matrix which includes bile acid in solution. The entrapped solution gives some apparent adsorption but this can be removed by washing the fibers with water. This method differentiates between strong adsorption and reversible adsorption. This enables dietary vegetable fibers to be classified according to their bile acid adsorption capacity which may be useful in selecting fibers for clinical trials.


KEY WORDS: • dietary vegetable fiber • fiber preparation • bile acid adsorption • water holding capacity

Manuscript received 5 December 1975.





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