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Effects of Dietary Vitamin B-6 Levels and Exercise on Glutamic-pyruvic Transaminase Activity in Rat Tissues1

Linda H. Chen and Abby L. Marlatt

Department of Nutrition and Food Science, University of Kentucky, Lexington, Kentucky 40506

The effects of dietary vitamin B-6 levels and exercise on glutamic-pyruvic transaminase (GPT) activity was studied with 96 weanling male rats divided into 4 groups and receiving 1.0, 2.0, 4.0, and 8.0 mg pyridoxine/kg diet, respectively. Half of the rats in each group were exercised daily; the other half stayed inactive in the cage. After periods of 4, 8, 12, and 16 weeks, 24 rats, 3 from each treatment, were killed, and GPT activities of erythrocyte, plasma, and liver samples were determined. In all periods, GPT activities in erythrocytes and plasma were significantly higher with higher levels of pyridoxine. In liver, a significant increase in GPT activity was shown only at 4 and 12 weeks. Exercise did not affect GPT activity. Preincubation with pyridoxal phosphate in vitro stimulated the GPT activity in all samples. This suggests that the apoenzyme (GPT) was biosynthesized in excess of the amount of pyridoxal phosphate available at all levels of dietary pyridoxine tested.

KEY WORDS: • vitamin B-6 • glutamic-pyruvic transaminase • exercise

1 From Department of Nutrition and Food Science, University of Kentucky, Lexington, Kentucky. Paper no. 74-9-92 published with the approval of the Director of Kentucky Agricultural Experiment Station.

Manuscript received 1 July 1974.




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