Journal of Nutrition

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Journal of Nutrition Vol. 105 No. 3 March 1975, pp. 379-384
Copyright © 1975 by American Society for Nutrition
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Gluconeogenesis from L-Cysteine in the Perfused Rat Liver1, 2,

Ronald C. Simpson, Fredric W. Hill and Richard A. Freedland

Department of Physiological Sciences, School of Veterinary Medicine and Department of Nutrition, University of California, Davis, California 95616

The effects of dietary and hormonal treatments on the rate of gluconeogenesis from L-cysteine have been investigated in the perfused rat liver in situ. In order to demonstrate gluconeogenesis from L-cysteine, rats were fed either a 90% casein diet, or this diet with 2 or 4% cysteine, added in place of casein, and perfused in the fed state; or fed stock diet and starved 48 or 72 hours; or fasted and injected with cortisol. The net rate of gluconeogenesis (in µmoles/min/g liver) from cysteine in rats fed 4% cysteine was 0.24; in 48-hour starved rats it was 0.10; in 72-hour starved rats it was 0.16; and in the cortisol injected rats it was 0.23. When [U-14C]cysteine plus carrier cysteine (10 mM) was added as the substrate for gluconeogenesis in 72-hour starved rats; 3.9% of the label appeared in glucose. The above dietary and hormonal treatments stimulated gluconeogenesis from L-cysteine.

KEY WORDS: • gluconeogenesis • L-cysteine • liver perfusion • cortisol

1 Supported by Grant USPHS AM-04732 and a Procter and Gamble Fellowship.

2 A preliminary report was presented at the 58th Annual Meeting of the Federation of American Societies of Experimental Biology, 7–12 April 1974. Atlantic City, N.J. Federation Proc. 33, 2661. (Abstr.)

Manuscript received 21 October 1974.




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