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Production and Nutritional Evaluation of a High Lysine Baker's Yeast (Saccharomyces cerevisiae) in Rats^1,2,3,

Division of Nutrition, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232

A high lysine baker's yeast (HLBY) was prepared by a bioorganic process in which synthetic DL--aminoadipic acid was converted to lysine by incubation under appropriate conditions with commercial baker's yeast (BY). HLBY contained about 10% lysine on a dry weight basis or about three times as much lysine as BY. Under the fermentation conditions described employing DL-[6-¹⁴C]--aminoadipic acid as a substrate, about 94% of the radioactivity was recovered principally in terms of intracellular biosynthesized lysine, or residual extracellular L--aminoadipic acid or D--aminoadipic acid. At least 90% of the lysine in HLBY was available to support growth of weanling rats fed a 20% wheat gluten diet limiting in lysine, and it was demonstrated that supplementation of wheat flour with 2% HLBY doubled the protein efficiency ratio of the flour. The merits of HLBY as a protein supplement to improve the nutritional quality of cereal grain proteins are briefly discussed.

KEY WORDS: • lysine • -aminoadipic acid • wheat gluten • protein efficiency ratio

¹ Supported in part by National Institutes of Health Grant 5 R01AM14338. This work was conducted during the tenure of R. F. Florentino of a Fellowship grant from the US-Agency for International Development and the Philippine Government.

² Taken in part from a dissertation submitted by R. F. Florentino in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Vanderbilt University.

³ Presented in part at IX International Congress of Nutrition. September. 1972. Mexico City, Mexico.

⁴ Present address: Food and Nutrition Research Center, Herran, Manila. Philippines.

⁵ Address reprint requests to: Dr. H. P. Broquist. Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

Manuscript received 20 December 1973.