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Effect of Feeding "Carbohydrate-free" Diets on the Chick's Requirement for Methionine^{1 ,2 ,3}

School of Household Economics, University of Alberta, Edmonton, Alberta, Canada T6G 2E2

Experiments were conducted to study the effect of fat on the chick's requirement for methionine when fat was substituted isocalorically for carbohydrate in a semipurified diet in which protein was supplied by isolated soybean protein. Using either growth or energetic efficiency as the criterion, the chick's requirement for methionine was shown to remain unchanged when hydrogenated fat was substituted isocalorically for glucose, irrespective of the vitamin B-12 status of the chick. Thus, the increased requirement for vitamin B-12 which has been previously observed when fat was substituted isocalorically for glucose cannot be attributed to increased need for methionine, and may reflect a role for vitamin B-12 in fat metabolism.

KEY WORDS: • methionine • fat • "carbohydrate-free" nutrient requirement • chick

¹ Supported in part by a research grant from the National Research Council of Canada.

² The data were taken from a thesis submitted by Su Hoon Looi in partial fulfillment for the degree of Doctor of Philosophy at the University of Alberta.

³ Requests for reprints should be addressed to Ruth Renner, School of Household Economics, Edmonton, Alberta, T6G 2E2.

⁴ Present address: Malaysian Agricultural Research and Development Institute (MARDI), Serdang, Selangor, Malaysia.

Manuscript received 18 July 1973.