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Effect of Feeding "Carbohydrate-free" Diets on the Chick's Requirement for Vitamin B-12^{1 ,2 ,3}

School of Household Economics, University of Alberta, Edmonton, Alberta, Canada T6G 2E2

Experiments were conducted to study the effect of fat on the chick's requirement for vitamin B-12 when fat was substituted isocalorically for carbohydrate in a semipurified diet in which protein was supplied by isolated soybean protein and in which methionine was supplied in limited amounts. Using growth as the criterion, the chick's requirement for vitamin B-12 was shown to increase when hydrogenated fat was substituted isocalorically for glucose. Pair-feeding studies showed that vitamin B-12 stimulated growth of chicks fed either carbohydrate-containing or "carbohydrate-free" diets by stimulating appetite and not by increasing utilization of either protein or energy.

KEY WORDS: • vitamin B-12 • fat • nutrient requirement • "carbohydrate-free" • chick

¹ Supported in part by a research grant from the National Research Council of Canada.

² The data were taken from a thesis submitted by Su Hoon Looi in partial fulfillment of requirements for the degree of Doctor of Philosophy at The University of Alberta.

³ Requests for reprints should be addressed to Ruth Renner, School of Household Economics, Edmonton, Alberta, T6G 2E2.

⁴ Present address: Malaysian Agricultural Research and Development Institute (MARDI), Serdang, Selangor, Malaysia.

Manuscript received 18 July 1973.