QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Romsos, D. R. Articles by Leveille, G. A. Search for Related Content PubMed PubMed Citation Articles by Romsos, D. R. Articles by Leveille, G. A.

Effect of Dietary 1,3-Butanediol on in vitro Fatty Acid Synthesis and Malic Enzyme Activity in Rat Liver and Adipose Tissue^1,2,

Laboratory of Nutritional Biochemistry, Department of Animal Science, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801 and Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824

Diets containing graded levels of 1,3-butanediol (BD) substituted for approximately equal amounts of glucose energy were fed to rats. Body weight gain was not affected by addition of 10 parts (17% of dietary energy) BD to the diet; however, addition of 15 parts BD (25% of dietary energy) or mode did depress weight gain. Plasma ß-hydroxybutyrate and acetoacetate levels were elevated as dietary BD was increased. Increasing the BD content of the diet decreased plasma glucose, insulin and triglyceride levels. Plasma free fatty acids were increased by addition of BD to the diet. Hematocrit was increased slightly in rats fed the BD-containing diets and may have been related to the increased rate of urination associated with elevated plasma ketones. Hepatic rates of in vitro fatty acid synthesis were markedly depressed when BD was added to the diet. The in vitro rates of fatty acid synthesis in adipose tissue were not affected by dietary BD. Malic enzyme activity was depressed in both liver and adipose tissue only when high levels of BD were added to the diets. It is suggested that dietary BD affects hepatic fatty acid synthesis in the rat by its effect on the hepatic NADH/NAD⁺ ratio.

KEY WORDS: • liver • adipose tissue • fatty acid synthesis • malic enzyme • ketone bodies • triglycerides • butanediol

¹ Supported in part by Public Health Service Grant no. HL14677 from the Heart and Lung Institute. Michigan Agricultural Experiment Station Journal Article no. 6405.

² A preliminary report has appeared: Sasse, C. E., G. A. Leveille, D. R. Romsos and D. H. Baker 1972 Metabolic effects of feeding 1,3-butanediol to rats. J. Anim. Sci. 35: 223 (abstr.).

³ Present address: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Mich. 48824.

⁴ Present address: Department of Animal Science, Texas Tech. University, Lubbock, Texas 79400.

Manuscript received 4 June 1973.