QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schafer, D. F. Articles by Sorrell, M. F. Search for Related Content PubMed PubMed Citation Articles by Schafer, D. F. Articles by Sorrell, M. F.

Effects of Ethanol on the Transport of Manganese by Small Intestine of the Rat^1,2,3,

Liver Study Unit, Veterans Administration Hospital and the Department of Internal Medicine and Surgery, University of Nebraska Medical Center, Omaha, Nebraska 68105

Previously published studies have shown an increase of hepatic manganese in rats chronically fed ethanol. In this study both in vitro and in vivo experiments were conducted to demonstrate whether ethanol influences intestinal absorption of manganese and it also sought to separate the direct effects of ethanol from its metabolic effects on the transmural movement of manganese across the intestine. The transmural movement of manganese was measured in isolated loops of rat jejunum and compared to that obtained when the perfusate on the mucosal side of the tissue contained 3% ethanol. In in vivo studies, fasted rats were gavaged with 0.01 µCi ⁵⁴Mn in the presence of either water or ethanol solution (4 g/kg body weight). After 3 hours the livers were excised and counted for radioactivity. In the in vivo experiments, ethanol stimulated the absorption of manganese twofold as measured by the uptake of this metal in the liver. The results of the in vitro experiments demonstrate that ethanol stimulated the transmural movement of manganese by a factor of four. Pyrazole, an alcohol dehydrogenase inhibitor, was found to reduce ethanol-induced transport of Mn by 70% in the perfused jejunum indicating that the effects of ethanol are principally mediated by metabolism of ethanol in the gut.

KEY WORDS: • ethanol • manganese • intestine • liver

¹ Veterans Administration project no. 0804-04.

² Presented in part to the American Federation for Clinical Research, Atlanic City, N. J., April, 1973. Clin. Res. 21, 523 (abstr.).

³ Presented in part to the 7th Annual Conference on Trace Substances in Environmental Health, University of Missouri, Columbia, Mo., June, 1973. Volume 7, P. D. Hemphill, editor (abstr.).

⁴ Present address: Department of Surgery, University of New Mexico, Albuquerque, N. M. 87106.

⁵ This investigator was supported in part by an Academic Career Development Award, no. AM 70316 from the National Institute of Arthritis and Metabolic Diseases.

Manuscript received 5 July 1973.