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An Optimal Level of Inorganic Sulfate for the Diet of a Rat^1,2,

Department of Nutrition, University of Tennessee, Knoxville, Tennessee 37916

The level of added inorganic sulfate required by adult rats fed semipurified diets was determined by measuring the percentage of a test dose of 1-¹⁴C-methionine expired as ¹⁴CO₂ in a 3-hour period. 1-¹⁴C-Methionine was selected for determination of optimal dietary sulfate level after it was determined that three times more ¹⁴CO₂ was expired from 1-¹⁴C-methionine in 3 hours than from U-¹⁴C-cysteine. Based on the assumption that as dietary sulfate spared methionine the ¹⁴CO₂ expiration would decrease, the optimum level of dietary inorganic sulfate was found to be 0.02%.

KEY WORDS: • inorganic sulfur • sulfate • methionine

¹ Published by permission of the Dean, Tennessee Agricultural Experiment Station.

² Supported in part by Biomedical Sciences Grant FR 7088 from the General Research Support Branch, Division of Research Resources, Bureau of Health Professions Education and Manpower Training, National Institutes of Health.

Manuscript received 9 January 1973.

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