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Respiratory Control and Swelling in Rat Liver Mitochondria: Effect of 60Co Gamma Radiation and Injected Nutrient

Wesley D. Skidmore

Armed Forces Radiobiology Research Institute, Defense Nuclear Agency, Bethesda, Maryland 20014

The objective of this study was to compare the relative effects of three treatments with that of radiation on the lag time for mitochondrial swelling and the respiratory control during oxidation of succinate. The three treatments were dietary supplementation with ethyl linoleate, time of feeding, and an intraperitoneal injection of a soluble diet. Treated and untreated rats from each of the eight different treatment groups were bilaterally exposed to 1000 rads of 60Co gamma rays. Liver mitochondria were collected from the 16 different groups 24 hours later for assay. Iron-induced mitochondrial swelling was used as an index of structural integrity. Respiratory control for mitochondrial oxidation of succinate in the presence of adenosine diphosphate was selected as an index of functional integrity. Results indicate that the intraperitoneal injection of a soluble diet significantly increased the lag time for swelling in every group given the injection in comparison with uninjected ones. In the assay for respiratory control, either whole-body irradiation or injection of a soluble diet significantly increased the respiratory control values above those for untreated ones. The significant effects were consistently observed in all treated groups.

KEY WORDS: • mitochondria • radiation • nutrition

Manuscript received 18 September 1972.




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