Journal of Nutrition

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Young, M. L.
Right arrow Articles by Seward, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Young, M. L.
Right arrow Articles by Seward, C. R.

Effect of Protein and Methionine on Vitamin a Liver Storage in Rats Fed DDT1

M. L. Young, G. V. Mitchell and C. R. Seward

Division of Nutrition, Food and Drug Administration, Department of Health, Education, and Welfare, Washington, D. C. 20204

The interrelationships among dietary protein, DDT, vitamin A, and DL-methionine were investigated in male weanling Holtzman rats fed purified diets, with either casein or soybean isolate as the protein source, for 4 weeks. Each protein was fed in the basal diet at a level of 10 or 20% with the following variation:

A) basal,
B) basal + 0.4% DL-methionine,
C) basal + 200 ppm DDT, and D) basal + 0.4% DL-methionine + 200 ppm DDt.
At the level of either 10 or 20% soy protein, DDT decreased the storage of vitamin A in the liver by 32%; the addition of methionine did not counteract this effect. At the 20% level of casein, storage of vitamin A in the liver was decreased 42% in rats fed DDT; however, in rats fed methionine plus DDT, there was only a 16% decrease in liver vitamin A as compared with the control rats (basal diet A). Methionine caused a significant increase in the amount of DDT, DDE, and DDD stored in the liver when given with either level of soy or with the 10% level of casein; at the 20% level of casein, methionine had no effect. These studies demonstrate that the degree of toxicological stress due to DDT exposure depends in large measure on the quality and quantity of dietary protein.

KEY WORDS: • protein • vitamin A storage • DL-methionine • DDT and its metabolites

1 A preliminary report of this work was given at the Fifty-fifth Annual Meeting of the Federation of American Societies for Experimental Biology, Chicago, Ill., April, 1971, Federation Proc. 30: 577 (abstr.).

Manuscript received 24 April 1972.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Copyright © 1973 by American Society for Nutrition