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Transketolase Activity in Tissues of Uremic Rats¹

Schools of Public Health and Medicine, University of California, Los Angeles, and Medical and Research Services, VA Wadsworth Hospital Center, Los Angeles, California

The activity of the enzyme transketolase (TK) was measured in rats made uremic by surgical destruction of renal tissue. These uremic animals together with sham-operated control rats were pair-fed diets containing 6%, 18% or 23% protein. Pair-fed rats were sacrificed when the urea clearance of the uremic rat was below 0.5 ml/minute. TK was measured in red cells, liver and brain. The inhibitory effect of uremic plasma on TK was determined by incubating red cell hemolysates from controls with plasma from control and uremic rats. TK activity of erythrocytes was higher in uremic than in control rats. TK from either liver or brain did not differ in control and uremic animals. No inhibitory effect of plasma from uremic rats on TK or erythrocytes was observed.

KEY WORDS: • enzymes • transketolase • uremia

¹ Supported in part by grants from the National Institutes of Health no. AM-15197 and no. 43-65-953.

² Taken in part from a dissertation submitted to the Graduate School of the University of California, Los Angeles, by Ofelia V. Dirige in partial fulfillment of the requirements for the degree of Doctor of Public Health.

³ Clinical Investigator, Veterans Administration.

Manuscript received 2 July 1973.