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Journal of Nutrition Vol. 102 No. 8 August 1972, pp. 1039-1044
Copyright © 1972 by American Society for Nutrition
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Effects of Dietary Cholesterol on the Activity of Some Carbohydrate Metabolism Enzymes in the Liver of Rats1

Alan C. Tsai and I. A. Dyer

Washington State University, Pullman, Washington 99163

The effect of cholesterol-cholic acid feeding on the activity of several carbohydrate metabolism enzymes in the liver of rats was studied. Glucose-6-phosphate dehydrogenase, NADP-malic enzyme, hexokinase, glucokinase and pyruvate kinase activities were determined in the hepatic tissue from rats which were fed the following: a) sucrose diets containing 0 or 1% cholesterol + 0.5% cholic acid for 12 weeks and then killed while being fed ad libitum or refed after a period of starvation; b) sucrose or starch diets with or without cholesterol-cholic acid supplementation for 12 weeks and then killed in a fed state; and c) sucrose diets with or without cholesterol-cholic acid supplementation for 12 days and killed in a fed state. The results show that liver glucokinase, glucose-6-phosphate dehydrogenase and NADP-malic enzyme activities were significantly reduced by cholesterol-cholic acid feeding under all of the conditions tested. Hexokinase and pyruvate kinase were not significantly changed except when the rats were refed after a period of starvation. A dietary regimen of fasting and refeeding elevated the activity of these two enzymes. The alterations in enzyme activities are interpreted as regulatory effects exerted by dietary cholesterol-cholic acid supplementation.

KEY WORDS: • dietary cholesterol • liver enzymes • glucose-6-phosphate dehydrogenase • NADP-malic enzyme • glucokinase • pyruvate kinase • cholesterol

1 Scientific Paper no. 3807. College of Agriculture Research Center, Washington State University, Project 0072.

Manuscript received 24 January 1972.




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