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Effect of Essential Fatty Acids on the Fatty Acid Synthesis in Epididymal Fat Cells of the Rat¹

Department of Physiological Chemistry, College of Medicine, Ohio State University, Columbus, Ohio 43210

The effect of dietary essential fatty acids (EFA) on lipogenesis in epididymal fat cells from glucose was investigated in an in vitro system. Adipocytes from rats fed a fat-free diet compared to those on a 5% corn oil diet incorporated eight to ten times more radioactivity from uniformly labeled glucose into fatty acids and also twofold more radioactivity into carbon dioxide. The rate of ¹⁴C-glycerol synthesis in adipocytes was the same in the two groups of rats. Only diets containing linoleate in the form of linoleate or corn oil markedly suppressed hyperlipogenic activity, and diets containing methyl oleate or hydrogenated coconut oil had little effect. These results indicate that dietary linoleate plays a regulatory role in fatty acid synthesis using glucose as the substrate.

KEY WORDS: • linoleate • adipocytes • lipogenesis • glucose

¹ Taken from the Ph.D. dissertation of Julie Y. Tsai Du. Department of Physiological Chemistry, College of Medicine, Ohio State University, June 1970.

² Present address: Department of Biochemistry, School of Medicine, University of Louisville, Louisville, Kentucky 40201.

Manuscript received 2 July 1971.