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Divalent Cation Uptakes by Rabbit Aorta — Interaction with Phospholipids¹

Nuclear Medicine Service, Veterans Administration Hospital, Brooklyn, New York 11209

Aortic intima-media strips prepared from normal and cholesterol-fed rabbits were separately incubated with 10^-4 M radioisotopic solutions of Mn²⁺, Ca²⁺, Cd²⁺, Zn²⁺ and Hg²⁺. Manganese uptake by intima-media strips was the lowest and zinc and mercury uptakes the highest. The ratios of the total amount (nanogram-atoms) of each metal retained by 100 mg of normal wet tissue using Mn²⁺ uptake as unity are: Mn:Ca:Cd:Zn:Hg = 1:3:7:13:14. No marked differences were noted in the metal uptakes by aortic strips between normal and cholesterol-fed rabbits. However, Zn²⁺ and Cd²⁺ uptakes of the cholesterol-fed rabbits appeared to be lower. When pairs of metal ion species were tested, marked competition between them for tissue uptake was noted.- Mercury inhibited Zn²⁺ uptake sharply, but Zn²⁺ had no effect on Hg²⁺ uptake. Zinc²⁺ and Cd²⁺ uptakes were mutually inhibitory, and Cd²⁺ and Zn²⁺ at higher concentrations were inhibitory to Ca²⁺ uptake. Phosphatidylserine and, to a lesser degree, phosphatidylethanolamine added to the incubation medium strikingly inhibited Zn²⁺ uptake by normal aortic strips. By contrast, phosphatidylcholine had no inhibitory effect on Zn²⁺ uptake. The significance of these results and their possible relationship to the pathogenesis of atherosclerosis is indicated.

KEY WORDS: • atherosclerosis • aortae • trace metal uptake • phospholipid cation interaction • metal ion competition

¹ Supported by Veterans Administration research funds.

Manuscript received 28 September 1971.