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Liver and Kidney Gluconeogenic and Lipogenic Enzyme Activities and Synthesis of Glucose by Kidney Cortex Slices in Rats Fed 1,3-Butanediol-containing Diets¹

Department of Biochemistry, University of Nebraska College of Medicine and V. A. Hospital, Omaha, Nebraska 68105

The activities of gluconeogenic and lipogenic enzymes in both liver and kidney from rats fed diets containing 1,3-butanediol (BD) for 5 weeks were examined. Pyruvate carboxylase and phosphoenolpyruvate carboxykinase activities were increased in both liver and kidney of rats fed BD-containing diets. There were no differences in liver malic, hexose monophosphate shunt, and isocitric dehydrogenase activities of BD-fed rats. In kidney a slight decrease in isocitric dehydrogenase activity of rats fed BD was observed. Glucose synthesis by rat kidney cortex slices from pyruvate and malate was greatly increased in rats fed BD-containing diets. Octanoylcarnitine decreased glucose synthesis from pyruvate in control but not in rats fed BD. Both acetylcarnitine and octanoylcarnitine increased glucose synthesis from malate in both control and BD-fed rats. The importance of feeding BD as a calorie source in relationship to gluconeogenesis is discussed.

KEY WORDS: • lipogenesis • gluconeogenesis • 1,3-butanediol

¹ Supported by grants from Celanese Chemical Company and National Institutes of Health (Grant no. 13,782).

Manuscript received 4 October 1971.