Journal of Nutrition

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Journal of Nutrition Vol. 102 No. 4 April 1972, pp. 489-494
Copyright © 1972 by American Society for Nutrition
This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Horn, G. W.
Right arrow Articles by Featherston, W. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Horn, G. W.
Right arrow Articles by Featherston, W. R.

Influence of Level and Source of Nitrogen Intake on Liver Glutamine Synthetase Activity in the Chick1

G. W. Horn2 and W. R. Featherston

Department of Animal Sciences, Purdue University, Lafayette, Indiana 47907

Studies were conducted with chicks on the influence of level and source of dietary nitrogen on hepatic glutamine synthetase activity. Chicks fed a 75% isolated soybean protein (ISP) diet for 10 days exhibited a fourfold increase in enzyme activity as compared with chicks fed a 25% ISP diet. Glutamine synthetase activity increased threefold during the first 24 hours chicks were fed the 75% ISP diet, remained at about this level for days 2 and 3 and increased slightly at day 4. The addition of 25% ISP or its nitrogen equivalent as diammonium citrate, glutamic acid or diammonium citrate + glutamic acid resulted in a significant increase (P < 0.05) in enzyme activity over that of chicks fed the 25% ISP diet. Feeding the diet containing both diammonium citrate and glutamic acid resulted in a moderate increase in enzyme activity over that observed when an isonitrogenous amount of diammonium citrate was fed singly. It is suggested that both substrates, ammonia and L-glutamate, act synergistically in the regulation of glutamine synthetase; a possible role of transdeamination as a mechanism involved in the regulation of glutamine synthetase in the chick is discussed.

KEY WORDS: • glutamine synthetase • nitrogen • liver

1 Journal paper no. 4565, Purdue University Agricultural Experiment Station. Supported in part by Public Health Service Research Grant AM 11487 from the National Institute of Arthritis and Metabolic Diseases.

2 Present address : Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30601.

Manuscript received 30 September 1971.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]