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Effect of Histidine and Ascorbic Acid on the Absorption and Retention of ⁵⁹Fe by Iron-depleted Rats

U. S. Plant, Soil, and Nutrition Laboratory, Soil and Water Conservation Research Division, Agricultural Research Service, U.S. Department of Agriculture, Ithaca, New York 14850

The effect of histidine and ascorbic acid on the retention of a single oral dose of ⁵⁹Fe by iron-depleted rats was determined by counting radio-activity of the rats in a "whole-body" counter. Histidine increased ⁵⁹Fe retention if the ⁵⁹Fe and histidine were mixed together prior to administering them to the rats; however, if histidine was fed and ³⁹FeCl₂ was administered 24 hours later by stomach tube, ⁵⁹Fe retention did not increase. Ascorbic acid increased ⁵⁹Fe retention either when it was mixed with the ⁵⁹Fe or when it was added to the diet.

KEY WORDS: • histidine • ascorbic acid • iron absorption

Manuscript received 7 July 1971.