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Department of Foods and Nutrition, Purdue University, Lafayette, Indiana 47907
Young adult human subjects consumed 8.0, 7.0, 6.0 and 5.0 g of nitrogen per day from rice, milk and wheat flour which provided 50, 25 and 25%, respectively, of the nitrogen in each treatment. Mean daily nitrogen balances decreased from + 0.85 to + 0.12 g. The regression of nitrogen balance on nitrogen intake was significant (P < 0.05) due primarily to the effect on urinary nitrogen (P < 0.01). Both 8.0 and 7.0 g of dietary nitrogen from these sources permitted sufficient nitrogen retention to compensate for integumental losses whereas 6.0 g was the minimal amount required for maintenance of nitrogen equilibrium. Therefore, 44 g of protein per day (equivalent to 0.6 g/kg of body weight) were adequate, 38 g (0.5 g/kg) met minimal requirements and 32 g (0.4 g/kg) were inadequate. The combination of 241 g of rice, 73 g of wheat flour and 27 g of nonfat dry milk that furnished 6.0 g of nitrogen contained 2.01 g of lysine, 1.68 g of sulfur-containing amino acids and 0.39 g of tryptophan.
KEY WORDS: protein requirements lysine methionine tryptophan rice milk wheat
1 From Purdue University Agricultural Experiment Station and School of Home Economics, Department of Foods and Nutrition, Lafayette, Indiana 47907. Journal paper 4682.
2 Supported in part by U. S. Public Health Service Grant AM 08533.
Manuscript received 8 March 1972.
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