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Carcass Amino Acids of the Rat under Conditions of Determination of Net Protein Utilization

Division of Food Technology and Nutrition, American University of Beirut, Beirut, Lebanon

Carcass amino acids were determined in rats under the experimental conditions of net protein utilization determination (NPU). Diets based on gluten, chickpea, casein and egg were fed at different concentrations such that the levels of utilizable protein (NDpJ%) were similar. At the end of the 10-day experimental period the amino acid compositions of the rat carcasses were determined together with those of the nonprotein and zero-day control groups. Tryptophan was also determined by ion-exchange chromatography following alkaline hydrolysis in vacuo. Except for increases in glycine and proline concentrations between the zero-day control group and the test groups, all other amino acid compositional differences were small and were only of the same order as the differences produced between replicate analyses of the same sample. Thus the underlying assumption of the NPU carcass analytical producedure that amino acid composition of the carcass is unaffected by the diet, is justified.

KEY WORDS: • body composition • amino acids • net protein utilization

^* Present address: Department of Nutrition and Food, University of Massachusetts, Amherst, Mass. 01002.

Manuscript received 22 March 1971.

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