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Effect of Protein Deprivation of Male Weanling Rats on the Kinetics of Hepatic Microsomal Enzyme Activity^1,2,

Department of Biochemistry and Nutrition, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061

The effect of protein deficiency in male weanling rats on the activity of the hepatic microsomal enzyme system was studied. Animals were divided into three groups and were fed for 15 days either a semipurified diet containing 5% casein (group 1), 20% casein pair-fed to the 5% casein group (group 2) or 20% casein fed ad libitum (group 3). The liver weight, as a percentage of body weight, was higher in group 1. For group 1 animals, microsomal protein and DNA, when expressed per gram of liver, were approximately one-half the contents of groups 2 and 3. Maximal velocities for ethylmorphine (EM) and aniline (AN) were lower in group 1, whether these rates are expressed per milligram microsomal protein, per gram liver or per 100 g body weight. A comparison of these methods of expression suggested that the diminution of enzyme velocities for the low protein group is related both to a lower DNA content per gram tissue and to a loss of specific enzymic activity per milligram microsomal protein. The K_m for ethylmorphine was lower and that for aniline was higher in group 1 animals. Restriction of food intake affected enzyme kinetic parameters similar to protein deficiency but to a significantly lesser degree. Alternative explanations for this effect of protein deficiency are discussed and an involvement of phospholipid is suggested.

KEY WORDS: • microsomal enzymes kinetics • protein deficiency • ethylmorphine • aniline

¹ Supported in part by Public Health Service Research Grant no. 1 R01 ES0036 from the National Institutes of Health, Division of Environmental Sciences.

² Presented in part at American Society of Pharmacology and Experimental Therapeutics Meetings, Federation of American Societies for Experimental Biology, Chicago, Illinois, April, 1971; Federation Proc. 40: 440 (abstr.).

³ This paper represents portions of a thesis to be submitted toward partial fulfillment of the requirements for the Ph.D. degree by Marcel U. K. Mgbodile.

Manuscript received 26 July 1971.

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