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Studies on Hepatic Polyribosomes and Protein Synthesis in Rats Force-fed a Protein-free Diet¹

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213

Young rats were tube-fed a protein-free diet following a 2-day fast. After 5 hours the fasted rats that received the protein-free diet, in comparison with those that received water, revealed a shift in hepatic polyribosomes (free and membrane-bound polyribosomes) toward heavier aggregates with fewer lighter units, and in vitro and in vivo hepatic protein synthesis were enhanced. Plasma insulin levels were increased in the experimental animals. In other experiments, young rats were force-fed a complete or protein-free diet for 1 day (three feedings) and killed the following morning (16 hours after the last feeding). The animals force-fed the protein-free diet revealed a shift in hepatic polyribosomes toward heavier aggregates (deoxycholate-treated postmitochondrial supernatants), and in vitro and in vivo hepatic protein synthesis were enhanced in comparison to animals force-fed the complete diet.

Manuscript received 8 March 1971.