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Journal of Nutrition Vol. 101 No. 3 March 1971, pp. 355-361
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Influence of Feeding Cottonseed Oil to Laying Hens on the Lipovitellins of Their Eggs1,2,

Robert John Evans, Doris H. Bauer and Cal J. Flegal

Departments of Biochemistry and Poultry Science, Michigan State University, East Lansing, Michigan 48823

Lipovitellins were isolated from normal eggs and from eggs laid by hens fed crude cottonseed oil ("cottonseed" lipovitellin). Cottonseed lipovitellin contained 23% lipid compared to 17% in normal lipovitellin, and the lipids of cottonseed lipovitellin contained more stearic acid and less oleic acid than those of normal lipovitellin. Amino acid compositions of the vitellins (proteins) were similar. Ether extracted 49% of the lipid from cottonseed lipovitellin compared to 16% of the lipid from normal lipovitellin. Progressive digestion with proteolytic enzymes released similar amounts of lipids and peptides from ether-extracted normal and cottonseed lipovitellins. Chromatography on a TEAE-cellulose column separated lipovitellins into {alpha}- and ß-lipovitellins. Normal lipovitellins were better separated than cottonseed lipovitellins, and the ratio of {alpha}-lipovitellin to ß-lipovitellin was smaller in normal than in cottonseed lipovitellins. The principal difference between normal and cottonseed lipovitellins appeared to be in the higher content of saturated fatty acids in the lipid, the larger amount of lipid bound on the outer surface of the molecule, and the higher proportion of {alpha}-lipovitellin in cottonseed lipovitellins than in normal lipovitellins.

1 Michigan Agricultural Experiment Station Journal Article no. 5135. Supported in part by Public Health Service Research Grant AM 10214 from the National Institute of Arthritis and Metabolic Diseases.

2 Presented in part at the 54th Annual Meeting of the Federation of American Societies for Experimental Biology, Atlantic City, New Jersey, April 12 to 17, 1970. Federation Proc. 29: 426 (abstr.).

Manuscript received 29 June 1970.




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