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Effect of Specific Amino Acids on in vitro Protein Synthesizing System from Rats¹

Veterans Administration Hospital and Department of Medicine, University of Wisconsin Medical School, Madison, Wisconsin

An in vitro amino acid incorporating system derived from weanling rat brain was used to study three phases of protein synthesis: 1) amino acid activation, 2) the incorporation of [¹⁴C]-leucine into protein from [¹⁴C]-leucine-tRNA, and 3) the incorporation of labeled amino acids into protein. The effect of single unlabeled amino acids added in excess was studied. Exogenous amino acids were added in certain specified increments to the concentrations endogenously present. It was demonstrated that of all the amino acids tested only valine, isoleucine, histidine, aspartate, methionine, and phenylalanine inhibited the incorporation of amino acids into protein. The site of inhibition appeared to be at the step of amino acid activation.

Manuscript received 9 February 1971.