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U.S. Army Medical Research and Nutrition Laboratory, Chemistry Division, Fitzsimons General Hospital, Denver, Colorado 80240
The flavin adenine dinucleotide (FAD) stimulation of the erythrocyte glutathione reductase (EGR) in the presence of FAD was studied during riboflavin depletion and the subsequent repletion of 65 rats. The percentage stimulation in the animals fed adequate amounts of riboflavin was found to be within a narrow range. Quantitative values cannot be ascertained in regard to the degree of riboflavin deficiency, but qualitative values were found for each animal. We have shown the percentage stimulation (activity coefficient) of the rat EGR in the presence of FAD to be a sensitive parameter of the measurement of the riboflavin status in the rat.