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Growth Effects of a Pyridoxol Analogue, 3-Hydroxy-2,4,5-trihydroxymethylpyridine¹

Department of Food Science, The Burnsides Research Laboratory, University of Illinois, Urbana, Illinois 61801

The compound, 3-hydroxy-2,4,5-trihydroxymethylpyridine (-hydroxypyridoxol), has been speculated to be a possible precursor in the biosynthesis of vitamin B₆. To test this hypothesis the compound was synthesized and purified from the reaction by-products through ion-exchange column chromatography. Bioautography showed that the isolated compound was free of pyridoxal and pyridoxol. This pyridoxol analogue showed about 5% vitamin B₆ activity for both Saccharomyces carlsbergensis and rats, and none for Lactobacillus casei. The activity of the compound for Saccharomyces carlsbergensis increased to about 10 or 20% when the yeast grew in the presence of 2 and 4 ng of pyridoxal, pyridoxol, or pyridoxamine per 10 ml of assay medium. When the pyridoxol analogue was added to a growing culture of a yeast mutant that excretes a fairly large amount of vitamin B₆, there was no increase in the amount of vitamin B₆ in the medium.

Manuscript received 24 June 1970.