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Journal of Nutrition Vol. 101 No. 1 January 1971, pp. 29-34
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Effect of Vitamin B6 Deficiency on Tissue Dehydrogenases and Fat Synthesis in Rats1

Dennis J. Sabo2, Ralph P. Francesconi and Stanley N. Gershoff

Department of Nutrition, Harvard School of Public Health, Boston, Massachusetts 02115, and U. S. Army Laboratories, Natick, Massachusetts 01760

The activities of glutamate (GDH), lactate (LDH), isocitrate (ICDH), malate (MDH), and glucose-6-phosphate (G6PDH) dehydrogenases were measured in liver, adipose tissue, and renal cortex and medulla homogenates from control and vitamin B6-deficient rats. G6PDH and LDH were significantly decreased in liver homogenates from deficient animals when compared to both ad libitum and pair-fed controls. Significant increases in all five enzymes were seen in adipose tissue homogenates from deficient rats only when compared to ad libitum controls. Except for an increase in kidney cortex G6PDH no significant changes were found in kidney enzymes of deficient rats. Insulin administration partially reversed the decreases in liver G6PDH and LDH activities. There were no significant differences in the ability of liver slices from fasted ad libitum-fed control and deficient rats to synthesize lipid from glucose or acetate. In fed animals synthesis of liver lipids from glucose but not acetate was significantly higher in slices from deficient rats. These data indicate that although carcass levels of fat are especially low in vitamin B6-deficient rats, their liver and adipose tissue may have a greater than normal capacity to synthesize fat when glucose is metabolically available in sufficient quantities.

1 Supported in part by U. S. Public Health Service Grant No. AM-03056 and the Fund for Research and Teaching, Department of Nutrition, Harvard School of Public Health.

2 Present address: Tufts University School of Medicine, Mallory Institute of Pathology, Boston, Massachusetts 02118.

Manuscript received 20 July 1970.




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