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Journal of Nutrition Vol. 100 No. 9 September 1970, pp. 1057-1062
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A New Approach to Erythrocyte Transketolase Measurement1

L. G. Warnock

Department of Biochemistry, Vanderbilt University, and the Research Division, Veterans Administration Hospital, Nashville, Tennessee 37203

A transketolase assay is described which distinguishes transketolase activity from the overall ribose-5-phosphate utilization. The transketolase enzyme can be saturated and hence the activity can be expressed as international units of enzyme activity: micromoles per hour per milliliter of whole blood. Data are given on thiamindeficient and pair-fed control animals showing that the transketolase enzyme is sensitive to thiamin deficiency. Normal and deficient values are indicated.


1 Supported in part by Public Health Service Research Grant no. AM-10200 from the National Institute of Arthritis and Metabolic Diseases and the Veterans Administration.

Manuscript received 1 April 1970.


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