Journal of Nutrition

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Journal of Nutrition Vol. 100 No. 9 September 1970, pp. 1053-1056
Copyright © 1970 by American Society for Nutrition
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Isolation and Identification of the Pyrimidine Moiety of Thiamin in Rat Urine Using Gas Chromatography-Mass Spectrometry1

William W. White, III, Willam H. Amos, Jr. and Robert A. Neal

Department of Biochemistry, Division of Nutrition, Vanderbilt University School of Medicine, Nashville, Tennessee 37203

The compound 2-methyl-4-amino-5-hydroxymethylpyrimidine has been isolated and identified as a metabolite of thiamin in the urine of rats receiving large oral doses of the vitamin.

1 Supported by U. S. Public Health Service Research Grant AM-10297.

Manuscript received 11 May 1970.




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