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Journal of Nutrition Vol. 100 No. 7 July 1970, pp. 739-748
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Separation of a Mouse Growth Inhibitor in Soybeans from Trypsin Inhibitors1,2,

D. J. Schingoethe3, S. D. Aust and J. W. Thomas

Departments of Dairy and Biochemistry, Michigan State University, East Lansing, Michigan 48823

Raw (unheated) soybean meal was subjected to numerous physical and chemical treatments in efforts to isolate a growth inhibitor fraction which was free of trypsin inhibitor (TI) activity. Each treatment fraction was added to the diet of growing mice and growth inhibitor activity determined by comparing their growth rates to growth rates achieved on an autoclaved soybean meal diet. A small molecular weight growth inhibitor was separated from TI by ion exclusion chromatography on a Sephadex G-50 column and partially characterized. This growth inhibitor fraction decreased weight gains and feed efficiencies without causing pancreatic enlargement. Evidence of the small size of this growth inhibitor includes removal by dialysis, retardation on a Sephadex G-25 column, and lack of detection by polyacrylamide-gel electrophoresis. High voltage electrophoresis at pH 3.5 indicated the presence of at least eight positively charged peptides in this fraction, one of which may be a glycopeptide.

1 Published with the approval of the Director of the Michigan Agricultural Experiment Station as Journal Article No. 4921.

2 Taken in part from a thesis submitted by the senior author to the Graduate School of Michigan State University, in partial fulfillment of the requirements for the Ph.D. degree.

3 Present address: Dairy Science Department, South Dakota State University, Brookings, South Dakota 57006.

Manuscript received 1 December 1969.




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