QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Perkins, E. G. Articles by Kummerow, F. A. Search for Related Content PubMed PubMed Citation Articles by Perkins, E. G. Articles by Kummerow, F. A.

Absorption by the Rat of Nonvolatile Oxidation Products of Labeled Randomized Corn Oil¹

The Burnsides Research Laboratory, Department of Food Science, The University of Illinois, Urbana, Illinois 61801

Randomized labeled corn oil was prepared by transesterification of corn oil with mono- and dilinolein prepared from ¹⁴C-labeled methyl linoleate. A portion of this oil was heated at 200° for 24 hours in the presence of air, saponified, the recovered fatty acids were subjected to partitioning between ethanol and hexane, and the oxidation products in the ethanol layer converted to the corresponding methyl esters. The oxidized methyl esters were fed to thoracic duct-cannulated rats, the lymph was collected, freed of lipid, and the lipids were subjected to thin-layer chromatography (TLC) and radioassayed. Furthermore, the lipids from representative tissue were subjected to TLC and to radioassay analysis. The results indicated that maximum absorption of the methyl esters of the nonvolatile oxidation products isolated from randomized heat-abused corn oil did not occur until 21 to 48 hours after it had been fed, and only 31.2% was absorbed and 18.7% metabolized within 48 hours after its administration. Approximately 7.6% of the radioactivity was found in the carcass, 1% in the liver, 5.9% in the gastrointestinal tract, 4.5% in the urine, and 62.8% in the feces.

Manuscript received 19 May 1969.