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A New Method for the Estimation of Percentage of Absorbed Nitrogen Retained by Rats and Pigs

Department of Nutrition, National Institute of Animal Industry, Chiba, Japan

Application of an indicator method to estimate the percentage of retained nitrogen relative to absorbed nitrogen was tried. The formula used for calculating the retained N (%) is 100 - (Iab/Nab) x (Nu/Iu) x R, in which Iab and Nab are, respectively, the digestible indicator content and digestible N content in the diet, calculated by multiplying each concentration in the diet by each digestibility estimated by the Cr₂O₃ indicator method. Nu and Iu are the concentrations of N and of the indicator, respectively, in the urine. The recovery of absorbed indicator is shown as R. By this method we can eliminate the quantitative collection of feces and urine. Potassium was chosen as the indicator. Potassium was easily absorbed but, in growing animals, its retention in the body was not negligible. For estimating R in growing animals formulae were obtained for the relationship between retained K and gain in weight. A close agreement was found between retained N (%) as determined by the K indicator method and by the standard total collection method.