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In Vitro Hemolysis as Related to Rat Erythrocyte Content of -Tocopherol and Polyunsaturated Fatty Acids

Laboratory of Nutrition and Endocrinology, National Institute of Arthritis and Metabolic Diseases, National Institutes of Health, Bethesda, Maryland 20014

The purpose of this study was to relate the amount of -tocopherol required in rat erythrocytes for prevention of hemolysis in vitro to their polyunsaturated fatty acid (PUFA) composition. Red cells of varying PUFA and -tocopherol contents were produced in rats and their hemolysis by dialuric acid determined. The erythrocyte concentration of -tocopherol required to prevent significant hemolysis ranged from 122 µg/100 ml packed cells when the peroxidizable index (PI) of the PUFA was lowest (linoleic acid-free diet), to 196 µg/100 ml when the PI was highest (5% corn oil in the diet). The average ratio µmoles -tocopherol/µmole PUFA to prevent 10% hemolysis was 0.00089, or one molecule of -tocopherol per 1100 molecules of PUFA. Over a wide range of blood -tocopherol concentrations, the erythrocyte content was about 0.45 of that in plasma.

Manuscript received 18 December 1969.