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Effect of Avitaminosis E on the Incorporation of ³⁵S-sulfate by Rat Tissue Components^{1, 2, 3,}

Department of Nutrition, Univiersity of Tennessee, Knoxville, Tennessee 37916

The effect of avitaminosis E upon the sulfation of cellular lipoprotein and its fractions was investigated by feeding diets low in inorganic sulfur and deficient in vitamin E or supplemented with -tocopheryl acetate. The incorporation of ³⁵S-sulfate into tissue components after the subcutaneous injection of Na₂³⁵SO₄ in isotonic saline was used as an index of sulfate utilization. An avitaminosis E-associated decrease in ³⁵S-sulfate incorporation into cellular lipoprotein, and its lipid and mucopolysaccharide fraction was obtained, whereas there was no effect on incorporation into the nucleotide, residual, or sulfate protein fraction. Determination of the incorporation of ³⁵SO₄ by lipoprotein fraction versus time demonstrated that the sulfate protein fraction became maximally labeled 0.5 hour after the injection of a test dose of ³⁵SO₄ while the mucopolysaccharide fraction was not maximally labeled until 24 hours after injection of ³⁵SO₄. The sulfate uptake of the most active fraction was not affected by avitaminosis E but the sulfate uptake of the least active fraction was reduced; therefore, the effect of avitaminosis E on sulfate utilization is shown to have specificity. Some of the physiological manifestations of avitaminosis E may be explained by the decreased fixation of sulfate by the sulfolipids.

² Presented in part at the 1966 Regional Meeting of the American Chemical Society, Louisville, Kentucky.

³ Supported in part by Public Health Service Research Grant No. AM-07800-03 from the National Institute of Arthritis and Metabolic Diseases.

Manuscript received 28 August 1969.