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Department of Animal Sciences, University of Kentucky, Lexington, Kentucky 40506
Amino acid compositions of diets, cecal contents, cecal bacteria and serum were determined in three permanently cecal-fistulated ponies, each fed corn, oats and barley diets. Amino acid profiles of hydrolysates of cecal bacteria from these ponies were similar. Higher molar percentages of several amino acids were observed in the cecal contents than in the diets. With a few exceptions, the observed molar ratios were consistent with partial conversion of feed nitrogen to bacterial protein. A significant linear dietary effect upon serum levels was observed for six amino acids. Except for leucine and phenylalanine, molar percentages of amino acids in the cecal contents and serum did not appear closely related.
2 Paper no. 69-5-112, published with the approval of the Director, Kentucky Agricultural Experiment Station.
3 The authors are indebted to H. H. Sutton for surgical services and E. Wachs, N. Tate and B. Lorrison for analytical assistance.
4 Present address: Department of Animal Science and Agricultural Biochemistry, University of Delaware, Newark 19711.
Manuscript received 15 September 1969.