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Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104
Samples of vitamin K isolated from the contents of bovine rumen were identified by chromatographic mobility, ultraviolet absorption spectra and mass spectra as menaquinones-10, 11, 12 and 13. The occurrence of these vitamins in the rumen supports the view that menaquinones-10, 11 and 12, isolated earlier from bovine liver, were bacterial in origin and were deposited in liver after intestinal absorption.
Manuscript received 29 September 1969.