QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Harada, N. Articles by Hamazima, Y. Search for Related Content PubMed PubMed Citation Articles by Harada, N. Articles by Hamazima, Y.

Influence of a Low Casein Diet on Malate Dehydrogenase Activity of Rat Liver Mitochondria and Homogenate Disrupted by Various Treatments

Laboratory of Nutrition, Chukyo Women's University, Ohbu, Chita-gun, Aichi-ken, Japan

It has been shown previously in this laboratory that the structure of rat liver mitochondria was changed by a low casein diet. In the present experiments, by measuring malate dehydrogenase activity, the degree of disruption by various destructive treatments on liver mitochondria from rats fed a low casein diet was investigated. The low casein diet contained 4% casein, and the control diet, 25%. Mitochondrial structure was disrupted by three methods: dilution with water, sonic disintegration, and addition of Triton X-100. Malate dehydrogenase activity per milligram of mitochondrial protein was higher in rats fed the low casein diet when the mitochondrial structure was disrupted by dilution with water and by addition of Triton X-100. However, when it was disrupted by sonic disintegration, the activity was almost the same in rats in both diet groups.